abi3730 xl automatic dna sequencer (Thermo Fisher)
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abi3730 xl automatic dna sequencer
Abi3730 Xl Automatic Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/automatic+dna+sequencer/Deoxyribonucleic+acid/pm41455074-74-13-18
Average 98 stars, based on 1 article reviews
Abi3730 Xl Automatic Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/automatic+dna+sequencer/Deoxyribonucleic+acid/pm41455074-74-13-18
Average 98 stars, based on 1 article reviews
abi3730 xl automatic dna sequencer - by Bioz Stars,
2026-10
98/100 stars
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Polymerase Chain Reaction:Article Title: Replication of a hepatitis C virus replicon clone in mouse cells Article Snippet: .. The PCR products were cloned into the pGEM-T Easy plasmid (Promega, Madison WI), and 12 clones of each fragment (4 from each cells line) were sequenced with an Article Title: Highly Tissue Specific Expression of Sphinx Supports Its Male Courtship Related Role in Drosophila melanogaster Article Snippet: .. PCR products were sequenced directly after purification [Qiagen (Valencia, CA) kit] on an Article Title: Degradation of Poly(ε-caprolactone) by thermophilic Streptomyces thermoviolaceus subsp. thermoviolaceus 76T-2 Article Snippet: To determine the nucleotide sequence of the 16S rRNA gene, genomic DNA was extracted from 5-day cultured cells using the QIAGEN® Genomic DNA Kit. .. A portion of the 16S rRNA gene was amplified by PCR as described by Nakajima et al. (Nakajima et al. ) and then sequenced directly on an Clone Assay:Article Title: Replication of a hepatitis C virus replicon clone in mouse cells Article Snippet: .. The PCR products were cloned into the pGEM-T Easy plasmid (Promega, Madison WI), and 12 clones of each fragment (4 from each cells line) were sequenced with an Plasmid Preparation:Article Title: Replication of a hepatitis C virus replicon clone in mouse cells Article Snippet: .. The PCR products were cloned into the pGEM-T Easy plasmid (Promega, Madison WI), and 12 clones of each fragment (4 from each cells line) were sequenced with an Article Title: Fc-binding protein exhibiting improved alkaline resistance, method for producing said protein, antibody adsorbent using said protein, and method for separating antibody using said antibody adsorbent Article Snippet: Coloration was stopped by adding 1 M phosphoric acid at 50 μL/well, and the absorbance at 450 nm was measured with a microplate reader (product of Tecan). (5) Approximately 2700 transformants were evaluated by the method of (4), and among these there were selected transformants expressing Fc-binding protein with increased stability compared to FcR35c. .. The selected transformants were cultured with 2YT liquid medium containing 50 μg/mL kanamycin, and an expression vector was prepared using a QIAprep Spin Miniprep kit (product of Qiagen Inc.). (6) The sequence of the polynucleotide region encoding Fc-binding protein that had been inserted into the obtained expression vector was supplied to a cycle sequencing reaction using a BigDye Terminator Cycle Sequencing FS Read Reaction Kit (product of Thermo Fisher Scientific) based on the chain terminator method, and the nucleotide sequence was analyzed with a fully Article Title: Recombinant FcγRII Article Snippet: .. Sequence analysis: DNA encoding improved recombinant FcγRIIb-a4F3 and its surrounding region from the recombinant vector was provided to cycle sequencing reaction using a Big Dye Terminator Cycle Sequencing FS Read Reaction kit (product of PE Applied Biosystems), based on the chain terminator method, and the base sequence was analyzed with a fully Article Title: Method for separating antibody, and method for testing on disease Article Snippet: (1) PCR was carried out using, as a template, an expression vector pET-FcR9_F containing a polynucleotide of SEQ ID NO: 10 which encodes the amino acid sequence of SEQ ID NO: 9 prepared in Example 1. .. Plasmids were extracted from the collected bacterial cells (transformants). (7) A cycle sequence reaction was performed using a BigDye Terminator v3.1 Cycle Sequencing Kit (manufactured by Life Technologies Corporation) based on the chain terminator method, and the nucleotide sequence of the polynucleotide encoding human FcγRIIIa and the surrounding region thereof in the resulting plasmid was analyzed by a fully Article Title: Recombinant FcγRII Article Snippet: .. Sequence analysis: DNA encoding improved recombinant FcγRIIa-m6 and its surrounding region from the recombinant vector was provided to cycle sequencing reaction using a Big Dye Terminator Cycle Sequencing FS Read Reaction kit (product of PE Applied Biosystems), based on the chain terminator method, and the base sequence was analyzed with a fully Sequencing:Article Title: Replication of a hepatitis C virus replicon clone in mouse cells Article Snippet: .. The PCR products were cloned into the pGEM-T Easy plasmid (Promega, Madison WI), and 12 clones of each fragment (4 from each cells line) were sequenced with an Article Title: Fc-binding protein exhibiting improved alkaline resistance, method for producing said protein, antibody adsorbent using said protein, and method for separating antibody using said antibody adsorbent Article Snippet: Coloration was stopped by adding 1 M phosphoric acid at 50 μL/well, and the absorbance at 450 nm was measured with a microplate reader (product of Tecan). (5) Approximately 2700 transformants were evaluated by the method of (4), and among these there were selected transformants expressing Fc-binding protein with increased stability compared to FcR35c. .. The selected transformants were cultured with 2YT liquid medium containing 50 μg/mL kanamycin, and an expression vector was prepared using a QIAprep Spin Miniprep kit (product of Qiagen Inc.). (6) The sequence of the polynucleotide region encoding Fc-binding protein that had been inserted into the obtained expression vector was supplied to a cycle sequencing reaction using a BigDye Terminator Cycle Sequencing FS Read Reaction Kit (product of Thermo Fisher Scientific) based on the chain terminator method, and the nucleotide sequence was analyzed with a fully Article Title: Recombinant FcγRII Article Snippet: .. Sequence analysis: DNA encoding improved recombinant FcγRIIb-a4F3 and its surrounding region from the recombinant vector was provided to cycle sequencing reaction using a Big Dye Terminator Cycle Sequencing FS Read Reaction kit (product of PE Applied Biosystems), based on the chain terminator method, and the base sequence was analyzed with a fully Article Title: Method for separating antibody, and method for testing on disease Article Snippet: (1) PCR was carried out using, as a template, an expression vector pET-FcR9_F containing a polynucleotide of SEQ ID NO: 10 which encodes the amino acid sequence of SEQ ID NO: 9 prepared in Example 1. .. Plasmids were extracted from the collected bacterial cells (transformants). (7) A cycle sequence reaction was performed using a BigDye Terminator v3.1 Cycle Sequencing Kit (manufactured by Life Technologies Corporation) based on the chain terminator method, and the nucleotide sequence of the polynucleotide encoding human FcγRIIIa and the surrounding region thereof in the resulting plasmid was analyzed by a fully Article Title: Fc-binding protein exhibiting improved alkaline resistance, method for producing said protein, antibody adsorbent using said protein, and method for separating antibody using said antibody adsorbent Article Snippet: TABLE 13 Composition Volume PCR product 2 μL 10 μM Forward primer 2 μL 10 μM Reverse primer 2 μL 5 × PrimeSTAR buffer 10 μL (Takara Bio, Inc.) 2.5 mM dNTPs 4 μL 2.5 U/μL PrimeSTAR HS 1 μL (Takara Bio, Inc.) H2O up to 50 uL (5) The V192p3 obtained in (4) was purified and then digested with restriction enzymes NcoI and HindIII and ligated with the expression vector pETMalE previously digested with restriction enzymes NcoI and HindIII (Japanese Unexamined Patent Publication No. 2011-206046), and the ligation product was used for transformation of E. coli BL21 (DE3). (6) The obtained transformants were cultured on LB medium containing 50 μg/mL added kanamycin. .. Plasmids were extracted from the collected cells (transformants). (7) The polynucleotide encoding Fc-binding protein and its surrounding regions in the obtained plasmids was supplied for cycle sequencing reaction using a BigDye Terminator v3.1 Cycle Sequencing Kit (product of Life Technologies Corp.) based on the chain terminator method, and the nucleotide sequence was analyzed with a fully Article Title: Recombinant FcγRII Article Snippet: .. Sequence analysis: DNA encoding improved recombinant FcγRIIa-m6 and its surrounding region from the recombinant vector was provided to cycle sequencing reaction using a Big Dye Terminator Cycle Sequencing FS Read Reaction kit (product of PE Applied Biosystems), based on the chain terminator method, and the base sequence was analyzed with a fully Cell Culture:Article Title: Fc-binding protein exhibiting improved alkaline resistance, method for producing said protein, antibody adsorbent using said protein, and method for separating antibody using said antibody adsorbent Article Snippet: Coloration was stopped by adding 1 M phosphoric acid at 50 μL/well, and the absorbance at 450 nm was measured with a microplate reader (product of Tecan). (5) Approximately 2700 transformants were evaluated by the method of (4), and among these there were selected transformants expressing Fc-binding protein with increased stability compared to FcR35c. .. The selected transformants were cultured with 2YT liquid medium containing 50 μg/mL kanamycin, and an expression vector was prepared using a QIAprep Spin Miniprep kit (product of Qiagen Inc.). (6) The sequence of the polynucleotide region encoding Fc-binding protein that had been inserted into the obtained expression vector was supplied to a cycle sequencing reaction using a BigDye Terminator Cycle Sequencing FS Read Reaction Kit (product of Thermo Fisher Scientific) based on the chain terminator method, and the nucleotide sequence was analyzed with a fully Expressing:Article Title: Fc-binding protein exhibiting improved alkaline resistance, method for producing said protein, antibody adsorbent using said protein, and method for separating antibody using said antibody adsorbent Article Snippet: Coloration was stopped by adding 1 M phosphoric acid at 50 μL/well, and the absorbance at 450 nm was measured with a microplate reader (product of Tecan). (5) Approximately 2700 transformants were evaluated by the method of (4), and among these there were selected transformants expressing Fc-binding protein with increased stability compared to FcR35c. .. The selected transformants were cultured with 2YT liquid medium containing 50 μg/mL kanamycin, and an expression vector was prepared using a QIAprep Spin Miniprep kit (product of Qiagen Inc.). (6) The sequence of the polynucleotide region encoding Fc-binding protein that had been inserted into the obtained expression vector was supplied to a cycle sequencing reaction using a BigDye Terminator Cycle Sequencing FS Read Reaction Kit (product of Thermo Fisher Scientific) based on the chain terminator method, and the nucleotide sequence was analyzed with a fully Recombinant:Article Title: Recombinant FcγRII Article Snippet: .. Sequence analysis: DNA encoding improved recombinant FcγRIIb-a4F3 and its surrounding region from the recombinant vector was provided to cycle sequencing reaction using a Big Dye Terminator Cycle Sequencing FS Read Reaction kit (product of PE Applied Biosystems), based on the chain terminator method, and the base sequence was analyzed with a fully Article Title: Recombinant FcγRII Article Snippet: .. Sequence analysis: DNA encoding improved recombinant FcγRIIa-m6 and its surrounding region from the recombinant vector was provided to cycle sequencing reaction using a Big Dye Terminator Cycle Sequencing FS Read Reaction kit (product of PE Applied Biosystems), based on the chain terminator method, and the base sequence was analyzed with a fully Purification:Article Title: Highly Tissue Specific Expression of Sphinx Supports Its Male Courtship Related Role in Drosophila melanogaster Article Snippet: .. PCR products were sequenced directly after purification [Qiagen (Valencia, CA) kit] on an Amplification:Article Title: Degradation of Poly(ε-caprolactone) by thermophilic Streptomyces thermoviolaceus subsp. thermoviolaceus 76T-2 Article Snippet: To determine the nucleotide sequence of the 16S rRNA gene, genomic DNA was extracted from 5-day cultured cells using the QIAGEN® Genomic DNA Kit. .. A portion of the 16S rRNA gene was amplified by PCR as described by Nakajima et al. (Nakajima et al. ) and then sequenced directly on an |